论文部分内容阅读
目的:观察玉米须(Stigma maydis or corn silk)对大鼠肝星状细胞(hepatic stellate cells)的增殖,并探讨其抗肝纤维化作用机制。方法:用链霉蛋白酶和胶原酶原位灌流,Metrizamide密度梯度离心分离大鼠肝星状细胞,用MTT比色法和ELISA法分别检测大鼠肝星状细胞增殖及Ⅰ型胶原的合成。结果:玉米须能明显抑制肝星状细胞的增殖及Ⅰ型胶原的合成,随着玉米须用量增加,抑制作用增强,呈明显的剂量效应关系(P<0.05)。结论:玉米须可通过抑制大鼠肝星状细胞增殖及Ⅰ型胶原合成而起到抗肝纤维化的作用。
Objective: To observe the proliferation of rat hepatic stellate cells by corn silk (Stigma maydis or corn silk) and to explore its anti-hepatic fibrosis mechanism. Methods: Rat hepatic stellate cells were isolated by in situ perfusion with pronase and collagenase, and then isolated by Metrizamide density gradient centrifugation. The proliferation of rat hepatic stellate cells and the synthesis of type Ⅰ collagen were detected by MTT assay and ELISA respectively. Results: Maize was able to significantly inhibit the proliferation of hepatic stellate cells and the synthesis of type Ⅰ collagen. With the increase of the amount of maize needles, the inhibitory effect was enhanced, showing a dose-response relationship (P <0.05). Conclusion: Corn fiber can play a role in anti-hepatic fibrosis by inhibiting hepatic stellate cell proliferation and type I collagen synthesis.