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通过在大豆基因组数据库中检索拟南芥At ABCG40在大豆中的同源基因,获得了Gm ABCG40基因序列。通过对Gm ABCG40基因编码的氨基酸序列及启动子序列进行生物信息学分析,结果表明:Gm ABCG40基因CDS序列全长4 284 bp,编码1 427个氨基酸。Gm ABCG40编码的蛋白为疏水性蛋白,具有多个N-糖基化位点、激酶磷酸化位点、N-豆蔻酰化位点、2个ATP/GTP结合位点基序A和1个速激肽家族信号。结构域分析表明Gm ABCG40含有2个核苷酸结合域与2个跨膜结构域,形成NBD1-TMD1-NBD2-TMD2结构,属于ABCG亚家族的成员。Gm ABCG40预测的启动子区域含有与激素、胁迫、光应答、胚乳表达和转录因子结合相关的顺式作用元件。系统进化分析表明Gm ABCG40与菜豆、红豆、木豆、百脉根等豆科植物亲缘关系较近。组织特异性表达分析结果显示Gm DABCG40在叶片中表达量最低,在根中表达量最高,推测其可能参与根中ABA的转运过程。
The Gm ABCG40 gene sequence was obtained by searching the soybean genome database for the Arabidopsis At ABCG40 homolog in soybean. The bioinformatics analysis of the amino acid sequence and promoter sequence of Gm ABCG40 gene showed that the CDS sequence of Gm ABCG40 gene was 4 284 bp in length and encoded 1 427 amino acids. The protein encoded by Gm ABCG40 is a hydrophobic protein with multiple N-glycosylation sites, kinase phosphorylation sites, N-myristoylation sites, two ATP / GTP binding site motifs A and one speed Kinin family signals. Domain analysis showed that Gm ABCG40 contains two nucleotide binding domains and two transmembrane domains, forming the NBD1-TMD1-NBD2-TMD2 structure, which belongs to the ABCG subfamily. The promoter region predicted by Gm ABCG40 contains cis-acting elements involved in hormones, stress, photo-response, endosperm expression and transcription factor binding. Phylogenetic analysis showed that Gm ABCG40 is closely related to leguminous plants such as kidney bean, red bean, pigeon pea and Lotus corniculatum. Tissue-specific expression analysis showed that Gm DABCG40 had the lowest expression in leaves and the highest expression in roots, suggesting that Gm DABCG40 may be involved in the ABA transport in root.