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用荧光光谱法研究了锌离子(Zn2+)与牛血清白蛋白(BSA)的作用机制。实验表明,Zn2+对BSA具有荧光猝灭作用,其猝灭方式为静态猝灭。求出了猝灭常数、结合常数及结合位点数。利用同步荧光光谱研究了Zn2+对BSA分子荧光光谱的影响。
The mechanism of zinc ion (Zn2 +) and bovine serum albumin (BSA) was studied by fluorescence spectroscopy. Experiments show that Zn2 + has a fluorescence quenching effect on BSA, and its quenching mode is static quenching. The quenching constant, the binding constant and the number of binding sites were calculated. The effect of Zn2 + on the fluorescence spectra of BSA was studied by synchronous fluorescence spectroscopy.