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目的为提高急性淋巴细胞白血病（ALL）微小残留检测的临床意义。方法建立竞争性定量聚合酶链反应（PCR）检测TCRVγI－Jγ基因重排技术并定量分析16例ALL病人残留白血病细胞。结果建立定量PCR方法的灵敏度为10-5水平。16例ALL缓解期残留白血病细胞为（427±364）×10-5病人。3例病人可检测出寡／亚克隆重排，白血病细胞定量为117×10-5、196×10-5及211×10-5水平，其中1例寡／亚克隆水平在病程中不断增加并导致3月后复发伴克隆演化。结论所建立定量PCR技术简便、快速、灵敏；定量检测ALL缓解期残留白血病有利于预后预测并可应用于寡／亚克隆的定量检测。
Objective To improve the clinical significance of detection of acute residual lymphocytic leukemia (ALL). METHODS: A competitive quantitative polymerase chain reaction (PCR) was used to detect the TCRVγI-Jγ gene rearrangement technique and quantitative analysis of residual leukemia cells in 16 patients with ALL. Results The sensitivity of the established quantitative PCR method was 10-5 levels. Residual leukemia cells in 16 ALL patients during remission were (427±364)×10-5 patients. The oligo/subclone rearrangement was detectable in 3 patients. The leukemic cells were quantified at 117×10-5, 196×10-5, and 211×10-5 levels. One of the oligo/subclone levels increased during the course of the disease. Lead to relapse with clonal evolution after 3 months. Conclusion The established quantitative PCR technique is simple, rapid and sensitive; Quantitative detection of residual leukemia in ALL during remission is beneficial to prognosis and can be applied to the quantitative detection of oligo/subclones.