论文部分内容阅读
目的通过观察胎盘组织匀浆上清(PTHS)对小鼠脾脏树突状细胞(DC)生物学特征的影响,探讨妊娠免疫耐受的机制。方法分离妊娠及正常BALB/C小鼠脾脏单个核细胞,诱导分化为树突状细胞,经PTHS作用后,流式细胞仪检测树突状细胞的表面标志和抗原摄取能力,MTT法检测其同种异基因淋巴细胞刺激能力,ELISA方法检测其产生的细胞因子水平。结果妊娠小鼠脾脏树突状细胞的生物学特征与正常对照小鼠比较差异无统计学意义,而经PTHS作用后的妊娠小鼠及正常对照小鼠的脾脏CD11b+树突状细胞明显降低,而CD8α+树突状细胞明显增高(P<0.01);树突状细胞的抗原摄取能力及刺激同种异基因的淋巴细胞反应的能力也明显低于正常小鼠(P<0.01);PTHS作用后的树突状细胞产生较高水平的IL-10和较低水平的IL-12,与对照组比较差异有统计学意义(P<0.01)。结论 PTHS可向下调节BALB/c小鼠脾脏树突状细胞的生物学特征,可能与妊娠免疫抑制有关。
Objective To observe the effect of placental homogenate supernatant (PTHS) on the biological characteristics of dendritic cells (DCs) in mice and explore the mechanism of immune tolerance in pregnancy. Methods The spleen mononuclear cells of pregnant and normal BALB / C mice were isolated and induced to differentiate into dendritic cells. After treated with PTHS, the surface markers and antigen uptake capacity of dendritic cells were detected by flow cytometry. Allogeneic lymphocyte stimulating ability, ELISA method to detect the level of cytokines produced. Results The biological characteristics of dendritic cells in spleen of pregnant mice showed no significant difference compared with that of normal control mice. CD11b + dendritic cells of pregnant mice and normal control mice after PTHS treatment were significantly decreased CD8α + dendritic cells were significantly increased (P <0.01). The ability of dendritic cells to uptake antigen and stimulate allogeneic lymphocyte reaction was also significantly lower than that of normal mice (P <0.01) Dendritic cells produced higher levels of IL-10 and lower levels of IL-12, compared with the control group, the difference was statistically significant (P <0.01). Conclusion PTHS can down-regulate the biological characteristics of spleen dendritic cells in BALB / c mice, which may be related to immunosuppression in pregnancy.