The central dogma (CD) of molecular biology is the transfer of genetic information from DNA to RNA to protein.Major CD processes governing genetic flow include the cell cycle,DNA replication,chromosome packaging,epigenetic changes,transcription,posttranscriptional alterations,translation,and posttranslational modifications.The CD processes are tightly regulated in plants to maintain genetic integrity throughout the life cycle and to pass genetic materials to next generation.Engineering of various CD processes involved in gene regulation will accelerate crop improvement to feed the growing world population.CRISPR technology enables programmable editing of CD processes to alter DNA,RNA,or protein,which would have been impossible in the past.Here,an overview of recent advancements in CRISPR tool development and CRISPR-based CD modulations that expedite basic and applied plant research is provided.Furthermore,CRISPR applications in major thriving areas of research,such as gene discovery (allele mining and cryptic gene activation),introgression (de novo domestication and haploid induction),and application of desired traits beneficial to farmers or consumers (biotic/abiotic stress-resilient crops,plant cell factories,and delayed senescence),are described.Finally,the global regulatory policies,challenges,and prospects for CRISPR-mediated crop improvement are discussed.