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AIM:To construct the recombinant pEgr-P16 plasmid forthe investigation of its expression properties in esophagealsquamous cell carcinoma induced by ionizing irradiation andthe feasibility of gene-radiotherapy for esophageal carcinoma.METHODS:The recombinant pEgr-P16 plasmid was constructedand transfected into EC9706 cells with lipofectamine.Westernblot,quantitative RT-PCR and flow cytometry were performedto study the expression of pEgr-P16 in EC9706 cells and thebiological characteristics of EC9706 cell line after transfectioninduced by ionizing irradiation.RESULTS:The eukaryotic expression vector pEgr-P16 wassuccessfully constructed and transfected into EC9706 cells.The expression of P16 was significantly increased in thetransfected cells after irradiation while the transfected cellswere not induced by ionizing irradiation.The induction ofapoptosis in transfection plus irradiation group was higherthan that in plasmid alone or irradiation alone.CONCLUSION:The combination of PEgr-P16 and irradiationcould significantly enhance the P16 exPression ProPerty andmarkedly induce aPoPtosis in EC9706 cells.These resultsrnay Iay an improtant eXperlment basis for gene radiotheraPy for esophageal earcinoma
AIM: To construct the recombinant pEgr-P16 plasmid forthe investigation of its expression properties in esophageal squamous cell carcinoma induced by ionizing irradiation and the feasibility of gene-radiotherapy for esophageal carcinoma. METHODS: The recombinant pEgr-P16 plasmid was constructed and transfected into EC9706 cells with lipofectamine . Western blot, quantitative RT-PCR and flow cytometry were performed to study the expression of pEgr-P16 in EC9706 cells and thebiological characteristics of EC9706 cell line after transfection induced by ionizing irradiation .RESULTS: The eukaryotic expression vector pEgr-P16 wassuccessfully constructed and transfected into EC9706 cells. The expression of P16 was significantly increased in the transfected cells after irradiation while the transfected cells are not induced by ionizing irradiation. induction ofapoptosis in transfection plus irradiation group was higherthan that in plasmid alone or irradiation alone .CONCLUSION: The combination of PEgr-P16 an d irradiationcould significantly enhance the P16 exPression ProPerty and markedly induce aPoPtosis in EC9706 cells. The results results in an Iy an improtant eXperlment basis for gene radiotheraPy for esophageal earcinoma