合成了T2毒素的人工抗原T2-BSA和[~3H]T2。用T2-BSA免疫家兔,获得高质量的抗血清,与[~3H]T2的亲和常数为3.2×10~9M~(-1),终滴度1:2000。测定水中T2的最小检测值为0.10ng(1.0ng/ml),测定血和尿的样品时最小检测值为0.20ng(2.0ng/ml)。批内精密度 CV<9％,批间精密度CV<12％。乙酰T2,HT2和T2三醇的交叉反应率为190％,20％和0.13％,T2四醇和疣孢菌素A无交叉反应。 The T2 toxin artificial antigens T2-BSA and [~ 3H] T2 were synthesized. Rabbits were immunized with T2-BSA to obtain high-quality antiserum with an affinity constant of 3.2 × 10 ~ 9M ~ (-1) with [~ 3H] T2 and a final titer of 1: 2000. The minimum detectable value of T2 in water was 0.10 ng (1.0 ng / ml) and the minimum detectable value of 0.20 ng (2.0 ng / ml) in blood and urine samples was measured. Intra-assay precision CV <9%, intra-assay precision CV <12%. The cross-reactivity rates of acetyl T2, HT2 and T2 triols were 190%, 20% and 0.13%, respectively. There was no cross reaction between T2 tetraol and Fusarium A.