论文部分内容阅读
目的 :研究谷氨酰胺对内毒素损伤的心肌的保护作用研究。方法 :取健康体重 (2 5 0± 30 )gSD雄性大鼠 2 2只随机分成 3组 ,正常对照组 (n =7) ,内毒素组 (n =7) ,谷氨酰胺 +内毒素组 (n =8) ,在Langendorff装置上用Krebs-Henseleit(KH)液对大鼠离体心脏行主动脉逆灌。连续记录心肌细胞单相动作电位 (MAP)、心肌收缩张力曲线 ,在相应的时点分别测定冠脉流出液中的超氧化物歧化酶 (SOD)活性和丙二醛 (MDA)含量。结果 :谷氨酰胺组对内毒素损伤引起的心肌细胞单相动作电位幅度减小及D2 0 延长、心肌张力降低、心率变慢等变化有明显的改善作用 ,同时可以明显逆转内毒素诱导的心肌SOD活性降低和MDA含量升高。结论 :谷氨酰胺对内毒素损伤的心肌有明显的保护作用 ,有机会成为一个有效防治内毒素性心肌损伤的药物。
Objective: To study the protective effects of glutamine on endotoxin-injured myocardium. Methods: Twenty two male SD rats weighing 250 ± 30 g were randomly divided into three groups: normal control group (n = 7), endotoxin group (n = 7), glutamine + endotoxin group n = 8). Rat aortic transfusions were performed on isolated rat heart using Krebs-Henseleit (KH) solution on a Langendorff apparatus. The monophasic action potential (MAP) and myocardial contractile tension curve of myocardial cells were recorded continuously. The activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in coronary effluent were measured respectively at the corresponding time points. Results: The glutamine group had a significant effect on reducing the amplitude of monophasic action potential of cardiomyocytes and the prolongation of D2 0, the decrease of myocardial tension and the slowing of heart rate induced by endotoxin injury, meanwhile, it could obviously reverse the changes of endotoxin induced myocardium SOD activity decreased and MDA content increased. CONCLUSION: Glutamine has a significant protective effect on endotoxin-injured myocardium and has the potential to become an effective drug for the prevention and treatment of endotoxic myocardial injury.