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目的:观察大鼠脑出血后血肿周围神经元线粒体内细胞色素C释放规律,血肿变化及中药复方血府逐瘀汤的干预作用。方法:实验于2003-10在湘雅医院中西医结合研究所进行。用Ⅶ胶原酶脑内立体定位注射诱导大鼠脑出血模型。免疫组织化学法检测神经元线粒体内细胞色素C的释放情况,同时测量不同时间点大鼠血肿的最大直径。结果:大鼠脑出血后血肿周围神经元于6h即有细胞色素C释放,出现胞浆染色的阳性细胞;1d后阳性细胞表达最多;3d后阳性细胞数减少;5d后阳性细胞继续减少。治疗组与模型组比较在1,3,5d时阳性细胞减少明显(P<0.01)。造模后血肿于1d达到最大,3d有所变小,5d变小明显;治疗组在6h与模型组比较血肿缩小明显(P<0.05),1,3,5d缩小更明显(P<0.01)。结论:脑出血后血肿周围神经元细胞色素C于6h即开始从线粒体释放,1d达高峰后下降;血府逐瘀汤能阻制细胞色素C的释放,同时促进血肿的吸收。
OBJECTIVE: To observe the cytochrome C release in the mitochondria and the changes of hematoma in rats with hematoma after intracerebral hemorrhage. METHODS: The experiment was performed at the Institute of Integrative Medicine of Xiangya Hospital in 2003-10. The intracerebral hemorrhage model induced by stereotaxic intracerebral injection of VII collagenase was used. Immunohistochemistry was used to detect the release of cytochrome C from mitochondria in neurons. The maximum diameter of hematoma was also measured at different time points. Results: After intracerebral hemorrhage, the neurons around the hematoma of rats developed release of cytochrome C at 6 h and showed positive cytoplasmic staining. After one day, the positive cells expressed the most; the number of positive cells decreased after three days; the positive cells continued to decrease after five days. Compared with the model group, the positive cells decreased significantly at 1, 3, and 5 days (P<0.01). The hematoma reached the maximum at 1d after modeling, decreased at 3d, and became smaller at 5d; the hematoma in the treated group was significantly reduced compared with the model group at 6h (P<0.05), and was significantly reduced at 1, 3, and 5d (P<0.01). . Conclusion: After intracerebral hemorrhage, cytochrome C began to release from the mitochondria 6 h after the onset of hematoma, and then decreased after 1d peak. Xuefu Zhuyu Decoction can inhibit the release of cytochrome C and promote the absorption of hematoma.