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目的:探讨M2型巨噬细胞对食管癌移植瘤脉管生成的影响及其可能机制。方法:应用PMA及IL-4将人淋巴瘤U937单核细胞诱导为M2型巨噬细胞。16只裸鼠分为2组,每组8只,注射与M2型巨噬细胞共培养的EC9706细胞或单独注射EC9706细胞;观察2组荷瘤裸鼠成瘤情况,采用原位杂交法检测移植瘤中VEGF和VEGF-C mRNA的表达,采用免疫组化方法检测2组移植瘤中VEGF和VEGF-C蛋白的表达,分别用CD31、D2-40标记2组移植瘤中血管和淋巴管,计算MVD与LMVD值。结果:M2型巨噬细胞与EC9706细胞共培养组裸鼠移植瘤中VEGF、VEGF-C mRNA及蛋白表达水平高于单独注射EC9706细胞的对照组(P<0.05),MVD及LMVD值亦高于对照组(P<0.05)。结论:M2型巨噬细胞可通过分泌VEGF、VEGF-C促进裸鼠食管癌移植瘤中血管、淋巴管的生成。
Objective: To investigate the effect of M2 macrophages on angiogenesis in esophageal carcinoma and its possible mechanism. Methods: U937 monocytes from human lymphoma were induced into M2 macrophages by PMA and IL-4. Twenty-six nude mice were divided into 2 groups with 8 mice in each group. EC9706 cells co-cultured with M2 macrophages or EC9706 cells were injected alone. The tumorigenicity of nude mice in 2 groups was observed. The in situ hybridization The expression of VEGF and VEGF-C mRNA were detected by immunohistochemistry. The expressions of VEGF and VEGF-C in two groups were detected by immunohistochemistry. The numbers of vascular and lymphatic vessels in two groups were marked by CD31 and D2-40 respectively MVD and LMVD values. Results: The expression of VEGF, VEGF-C mRNA and protein in the nude mice transplanted with M2 macrophages and EC9706 cells was higher than that of EC9706 cells alone (P <0.05), and the MVD and LMVD values were also higher Control group (P <0.05). Conclusion: M2 macrophages can promote the formation of blood vessels and lymphatic vessels in transplanted esophageal cancer in nude mice by secreting VEGF and VEGF-C.