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目的研究细胞内外La3+对急性分离大鼠海马神经元IA、IK电流的影响。方法运用全细胞膜片钳技术记录细胞内外施加La3+后IA、IK的变化。结果细胞外100μmol/L La3+使IA稳态激活曲线和稳态失活曲线半数电压向去极化方向移动,IA失活恢复时间延长,IA激活动力学抑制。细胞内100μmol/L La3+使IK稳态激活曲线左移,并减弱了细胞外La3+引起的IA稳态失活曲线右移程度。结论细胞内外La3+可能与IA、IK通道蛋白某些位点结合而发挥对通道蛋白的调控作用,细胞外La3+对IA通道蛋白的多个状态都有影响,并且细胞外La3+对IA稳态失活的部分作用可能通过第二信使实现。
Objective To study the effect of intracellular La3 + on IA and IK currents in acutely isolated rat hippocampal neurons. Methods The whole cell patch clamp technique was used to record the changes of IA and IK after intracellular and extracellular application of La3 +. Results The extracellular 100μmol / L La3 + shifted the steady-state activation curve and the half-value of steady-state inactivation curve toward depolarization. The recovery time of IA inactivation was prolonged and the kinetics of IA activation was inhibited. Intracellular 100μmol / L La3 + left IK steady-state activation curve shifted to the left, and weakened the extracellular La3 + induced rightward homeostatic IA inactivation curve. Conclusion La3 + may play a role in the regulation of channel proteins by binding to some sites of IA and IK channel proteins. Extracellular La3 + has effects on various states of IA channel proteins, and the extracellular La3 + inactivates IA Part of the effect may be achieved through the second messenger.