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目的:观察虎杖苷对大鼠脑出血损伤后脑脊液中兴奋性氨基酸动态变化和脑组织含水量的影响,探讨虎杖苷对脑出血损伤的治疗作用及其作用机制。方法:采用微透析-高效液相色谱法建立天门冬氨酸(Asp)、谷氨酸(G lu)的测定方法;用Ⅶ型胶原酶诱导建立大鼠脑出血模型,观察虎杖苷对大鼠脑出血后0,6,12,24,36,48,60,72,84,96,108 h脑脊液内天门冬氨酸、谷氨酸含量的动态变化,并断头取脑检测脑组织含水量。结果:天门冬氨酸和谷氨酸含量在大鼠脑出血后24 h内迅速升高,并于24 h达到最高,随后逐渐开始降低。虎杖苷治疗后,天门冬氨酸和谷氨酸含量均上升缓慢,分别在12~72,6~84 h与同时段模型组比较有显著性差异(P<0.01或P<0.05),84,96 h后与同时段模型组相比无显著性差异。与假手术组比较,模型组脑含水量明显较高,虎杖苷组则明显降低(P<0.01)。结论:虎杖苷能抑制脑出血后脑脊液中ASP,G lu含量升高,能明显抑制脑出血造成的脑水肿,表明虎杖苷抗脑出血损伤的作用机制可能与其抗脑出血后兴奋性氨基酸含量的升高有关。
Objective: To observe the effect of polydatin on dynamic changes of cerebrospinal fluid excitatory amino acids and brain water content after intracerebral hemorrhage in rats, and to explore the therapeutic effect of polydatin on cerebral hemorrhage injury and its mechanism. Methods: The method of determination of Asp and G lu was established by microdialysis-high performance liquid chromatography (HPLC). The model of intracerebral hemorrhage was induced by collagenase type Ⅶ. The changes of aspartate and glutamic acid contents in cerebrospinal fluid at 0, 6, 12, 24, 36, 48, 60, 72, 84, 96, 108 h after intracerebral hemorrhage were measured. Results: The content of aspartate and glutamate increased rapidly within 24 hours after intracerebral hemorrhage in rats and reached its peak at 24 hours, then gradually began to decrease. The content of aspartic acid and glutamic acid increased slowly after treatment with polydatin, and there was significant difference (P <0.01 or P <0.05) between the two groups at 12 ~ 72 and 6 ~ 84 h (P <0.01 or P <0.05) 96 h after the same period compared with the model group no significant difference. Compared with the sham operation group, the brain water content of the model group was significantly higher, while that of the corticosteroid group was significantly decreased (P <0.01). CONCLUSION: Polydatin can inhibit the increase of ASP and G lu in cerebrospinal fluid after intracerebral hemorrhage and inhibit cerebral edema caused by cerebral hemorrhage. It suggests that the mechanism of cediidin against intracerebral hemorrhage may be related to its anti-brain amino acid content Increased related.