论文部分内容阅读
目的探讨sel-1样基因(SEL1L)在胃癌组织中的表达及与临床病理因素之间的关系。方法应用免疫组织化学染色方法及原位杂交方法检测SEL1L在100例胃癌组织、20例非典型增生胃组织和30例正常胃黏膜组织中的表达。结果 SEL1L蛋白在上述组织中的阳性表达率分别为83.0%、85.0%和16.7%,胃癌组织及非典型增生组织中的表达率显著高于正常组(P<0.01);相对应的3组中SEL1L的信使核糖核酸(mRNA)的阳性表达率分别为90.0%、95.0%和10.0%,前2组的表达率显著高于正常组(P<0.01)。结论 SEL1L的过表达可能是胃癌发生的早期表现,检测胃癌中的SEL1L的表达对于评估胃癌的发生和发展具有重要意义。
Objective To investigate the expression of sel-1-like gene (SEL1L) in gastric carcinoma and its relationship with clinicopathological factors. Methods The expression of SEL1L in 100 cases of gastric cancer, 20 cases of atypical hyperplastic stomach and 30 cases of normal gastric mucosa were detected by immunohistochemistry and in situ hybridization. Results The positive rates of SEL1L protein in the above tissues were 83.0%, 85.0% and 16.7%, respectively. The expression rates of SEL1L protein in gastric cancer tissues and atypical hyperplasia tissues were significantly higher than those in normal controls (P <0.01) The positive rate of mRNA expression of SEL1L was 90.0%, 95.0% and 10.0%, respectively. The expression rates of SEL1L in the two groups were significantly higher than those in the normal group (P <0.01). Conclusion The overexpression of SEL1L may be an early manifestation of gastric cancer. Detecting the expression of SEL1L in gastric cancer is of great significance for evaluating the occurrence and development of gastric cancer.