目的观察所获得的增强子结合蛋白家族一个新基因ＨＰ８的３′┐非编码区（３′ＵＴＲ）对肝癌细胞生长的影响。方法将３′ＵＴＲ序列亚克隆至真核表达载体ｐＢＫＣＭＶ中，运用磷酸钙沉淀法转染肝癌细胞７７２１。结果观察到细胞的生长受到较明显抑制。结论表明该基因参与真核细胞转录调控，结合以前的实验结果（该基因在肝癌细胞中普遍高表达），提示该基因与细胞生长调控密切相关 Objective To observe the effect of the 3′UTR of a new gene, HP8, on the growth of hepatoma cells. Methods The 3′ UTR sequence was subcloned into the eukaryotic expression vector pBKCMV, and the hepatoma cells 7721 were transfected by calcium phosphate precipitation. As a result, it was observed that the cell growth was significantly inhibited. The conclusions indicate that this gene is involved in the transcriptional regulation of eukaryotic cells, combined with previous experimental results (the gene is generally highly expressed in hepatoma cells), suggesting that this gene is closely related to the regulation of cell growth.