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目的 研究鸦胆子油乳对肺癌细胞A549的抑制作用,并初步探讨其相关作用机制.方法 以不同终质量浓度的鸦胆子油乳(0.50,1.25,2.50 g·L-1)作用于肺癌细胞A549,并将其作为低、中、高3个质量浓度实验组,同时设置对照组(给予等量0.9％ NaCl).用4,6-二脒基-2-苯基吲哚(DAPI)染色法检测各组肺癌细胞A549凋亡的形态学变化,用噻唑蓝(MTr)法和免疫印迹法检测各组肺癌细胞A549增殖抑制情况及凋亡抑制蛋白Survivin表达情况.结果 干预24h后,对照组及低、中、高3个质量实验组肺癌细胞A549增殖抑制率分别为(0.31±0.19)％,(37.24±6.21)％,(49.33±5.96)％,(61.25±6.23)％,干预48 h后,增殖抑制率分别为(0.32±0.21)％,(58.14±6.33)％,(65.28±5.79)％,(73.46±5.37)％,干预72 h后,增殖抑制率分别为(0.38±0.23)％,(68.58±5.87)％,(76.35±6.02)％,(83.97±5.64)％.与对照组比较,实验组肺癌细胞A549增殖抑制率显著升高(P＜0.01),且随着鸦胆子油乳质量浓度增大和作用时间延长,肺癌细胞A549增殖抑制率呈逐渐升高趋势(P ＜0.05或P＜0.01).对照组和低、中、高3个质量浓度实验组Survivin蛋白灰度值分别为0.95±0.07,0.83±0.10,0.66±0.12,0.51±0.09;对照组和实验组比较,差异均有统计学意义(均P＜0.01),且随鸦胆子油乳质量浓度增加,Survivin蛋白表达量呈逐渐降低趋势(P＜0.01).结论 鸦胆子油乳可显著抑制肺癌细胞A549增殖,并诱导其凋亡,相关作用机制可能是其可有效抑制肺癌细胞A549中凋亡抑制蛋白Survivin的表达.“,”Objective To explore the inhibitory effect of bruceolic oil emulsion on lung carcinoma cell A549 and the related mechanism.Methods The lung carcinoma cell A549 managed by different final mass concentration bruceolic oil emulsion (0.50,1.25,2.50 g · L-1) were acted as low,medium,high concentration test groups,at the same time the control group was managed by equivalent 0.9％ NaCl.The morphological changes of lung carcinoma cell A549 in each group were detected by 4,6-diamino-2-phenyl indole (DAPI) staining.The proliferation inhibition and the expression of anti-apoptosis protein Survivin was detected by methyl thiazolyl tetrazolium (MTT) and Western blot respectively.Results At 24 h after intervention,the proliferation inhibition rates of control group and low,medium,high concentration test groups were (0.31 ± 0.19) ％,(37.24 ± 6.21) ％,(49.33 ± 5.96) ％,(61.25 ± 6.23) ％.The proliferation inhibition rates at 48 h after intervened were (0.32 ± 0.21) ％,(58.14 ± 6.33) ％,(65.28 ± 5.79) ％,(73.46 ± 5.37) ％.The proliferation inhibition rates at 72 h after intervened were (0.38 ±0.23)％,(68.58 ±5.87)％,(76.35 ±6.02)％,(83.97 ± 5.64)％.Compared with control group,the proliferation inhibition rate of lung carcinoma cell A549 in test groups increased significantly (P ＜ 0.01),and the proliferation inhibition rates showed a trend of gradually increasing with the increasing of bruceolic oil emulsion concentration and the extension of action time (P ＜ 0.05 or P ＜ 0.01).The Western blot detection results showed that,the Survivin protein grey value ratio of control group and low,medium,high concentration test groups were 0.95 ± 0.07,0.83 ± 0.10,0.66 ± 0.12,0.51 ± 0.09.Compared with control group,the expression of Survivin protein of lung carcinoma cell A549 in test groups decreased significantly (all P ＜ 0.01),and the expression of Survivin protein showed a trend of gradually decrease with the increasing of bruceolic oil emulsion concentration (P ＜ 0.01).Conclusion Bruceohc oil emulsion can inhibit the proliferation of lung carcinoma cell A549 effectively,and induce its apoptosis;the related action mechanism maybe be it can inhibit the expression of anti-apoptosis Survivin protein.