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[目的]本实验旨在观察热损伤所致胃人工溃疡的特点及康复新液对氩等离子凝固术诱导的大鼠胃人工溃疡的疗效。[方法]选取SD大鼠50只,雌雄各半,体重约200g,其中42只采用氩等离子(电凝指数A50,氩气流量2.0L/min,输出功率60W)灼烧大鼠胃黏膜造成胃人工溃疡,建立胃人工溃疡大鼠模型,8只仅做手术不灼烧胃黏膜。1周后共存活40只(其中仅做手术不灼烧胃黏膜的存活6只,灼烧胃黏膜的存活34只)。随机选取5只灼烧胃黏膜的大鼠解剖,取出胃组织肉眼观察溃疡形态及病理组织学检测。将剩余35只大鼠分为6组:正常对照组(6只);模型组(6只);阳性对照组(6只);康复新组大剂量组(6只);康复新中剂量组(6只);康复新小剂量组(5只)。治疗组给予康复新液灌胃,阳性对照组给予铝碳酸镁片灌胃。治疗1个月后,取各组动物血液及溃疡部位组织,检测血常规、组织病理学检查以及血清EGF、胃组织bFGF表达。[结果]胃溃疡模型中大鼠的胃组织可见灼烧部位胃壁有明显的凹陷、不平整、存在充血和出血的现象,但无穿孔。经康复新液治疗后明显好转,溃疡面缩小,无一例出血和红肿、溃疡面几乎平整。此外,康复新液治疗组较未治疗组,白细胞、红细胞、血小板均具有明显的升高趋势(P<0.05),而苏木精-伊红染色法检测发现,大剂量的康复新液处理胃溃疡的大鼠后,胃组织中炎症细胞浸润减少,炎症反应得到了明显的缓解。正常对照组大鼠EGF、bFGF的表达为弱阳性,溃疡模型组为强阳性表达。康复新液治疗组和西药治疗组均能较正常对照组明显增加大鼠血清中EGF和胃组织bFGF水平,差异具有统计学意义(P<0.05)。[结论]康复新液对大鼠胃人工溃疡有显著疗效,且呈一定剂量相关性。康复新液中、大剂量组对氩等离子诱导的大鼠胃人工溃疡疗效优于铝碳酸镁。康复新液能够通过升高胃组织内bFGF含量来促进氩等离子凝固术诱导的胃人工溃疡的愈合。
[Objective] The purpose of this experiment was to observe the characteristics of gastric ulcer induced by heat injury and the therapeutic effect of Kangfuxin on gastric ulcer induced by argon plasma coagulation in rats. [Methods] Fifty SD rats were selected and their body weight was about 200g. Forty-two SD rats were treated with argon plasma (coagulation index A50, argon flow rate 2.0L / min, output power 60W) Artificial ulcer, the establishment of gastric ulcer rat model, 8 only operation does not burn gastric mucosa. A total of 40 survived after 1 week (only 6 cases of gastric mucosal non-burning operation, 34 cases of burning gastric mucosal survival). Five randomly selected rats with gastric mucosa were dissected and their gastric tissues were removed by naked eye to observe the morphology and histopathology of the ulcer. The remaining 35 rats were divided into 6 groups: normal control group (6); model group (6); positive control group (6); Kang Fuxin group of high-dose group (6) (6); rehabilitation new small dose group (5). The treatment group was given Kangfuxin solution and the positive control group was given gavage with aluminum magnesium carbonate. After 1 month of treatment, blood and ulcer tissues of each group were taken for blood routine examination, histopathological examination, and expression of bFGF in serum EGF and gastric tissue. [Result] The gastric tissue of rats in gastric ulcer model showed obvious depression and uneven gastric wall in the burning area, with congestion and bleeding but no perforation. After the rehabilitation of new fluid significantly improved after treatment, ulcer surface reduced, no case of bleeding and swelling, ulcers almost flat. In addition, compared with the untreated group, the KFNF treatment group showed a marked increase tendency (P <0.05), while the hematoxylin-eosin staining method showed that the high dose of KFN treatment fluid After the ulcer rat, the infiltration of inflammatory cells in the gastric tissue decreased, and the inflammatory response was significantly alleviated. The expression of EGF and bFGF in the normal control group was weakly positive, and the expression of bFGF in the model group was strongly positive. Compared with the normal control group, the levels of EGF and bFGF in serum of rats in both KFXT group and western medicine group were significantly increased (P <0.05). [Conclusion] Kangfuxin solution has a significant curative effect on gastric ulcer in rats with a dose-related effect. Kangfuxin solution, high-dose group of argon plasma-induced gastric ulcer in rats better than the effect of magnesium aluminum carbonate. Kangfuxin solution can promote argon plasma coagulation-induced healing of gastric ulcer by increasing bFGF content in gastric tissue.