论文部分内容阅读
目的:观察不同剂量环磷酰胺(cyclophosphamide,CTX)预处理联合5 Gy60Co照射的半相合淋巴细胞输注(hap-loidentical lymphocyte infusion,HLI)对小鼠肝癌移植瘤的抑制作用。方法:以皮下接种Hepa1-6肝癌细胞的BABL/c×C57BL杂交F1代雌性小鼠(表型为H-2b/d)为受鼠,以BALB/c×C3H杂交F1代雌性小鼠(表型为H-2d/k)为MHC半相合的供者,分PBS组、CTX 80 mg/kg+5 Gy照射HLI组、CTX 200 mg/kg+5 Gy照射HLI组、CTX 300 mg/kg+5 Gy照射HLI组、5 Gy照射HLI组,每组5只小鼠;观察各组小鼠瘤块生长和大小,检测受鼠体内的嵌合状态及移植物抗宿主病(graft-versus host disease,GVHD)的发病情况。结果:80、200 mg CTX联合HLI组小鼠瘤体积小于PBS组[(1.25±0.24)、(1.38±0.31)vs(2.03±0.24)cm3,P<0.01],小鼠生存时间显著长于PBS组[48 d(39 d,55 d)、40 d(35 d,48 d)vs 35 d(18 d,39 d),P<0.05];80mg CTX联合HLI组的抑瘤作用强于单纯HLI组[(1.25±0.24)vs(1.76±0.40)cm3,P<0.05];300 mg CTX联合HLI组和单纯HLI组无明显抑瘤作用。各治疗组小鼠均未出现GVHD。80、200 mg CTX联合HLI组小鼠嵌合度低于300 mg CTX联合HLI组,且消失时间明显早于后者。结论:低剂量CTX联合输注经照射的半相合供者淋巴细胞可获得较好的抗小鼠肝癌移植瘤的作用,CTX剂量增加后抗肿瘤作用并未增强。
OBJECTIVE: To observe the inhibitory effect of haploidentical lymphocyte infusion (HLI) pretreated with cyclophosphamide (CTX) pretreatment and 5 Gy of 60Co on mice hepatoma transplanted tumor. Methods: Female BABL / c × C57BL hybrid F1 mice (H-2b / d phenotype) were inoculated subcutaneously into Hepa1-6 hepatoma cells, and BALB / c × C3H female F1 mice HLX group were treated with CTX 80 mg / kg + 5 Gy and CTX 200 mg / kg + 5 Gy were irradiated with CTX 300 mg / kg + The HLI group was treated with 5 Gy and the HLI group was treated with 5 Gy. The growth and size of the tumor were observed. The chimerism and graft-versus host disease , GVHD) incidence. Results: The tumor volume of mice in 80,200 mg CTX combined with HLI group was smaller than that in PBS group [(1.25 ± 0.24) vs (1.38 ± 0.31) vs (2.03 ± 0.24) cm 3, P <0.01] (P <0.05)]. The anti-tumor effect of 80 mg CTX combined with HLI group was stronger than that of HLI alone group (48 d, 39 d and 55 d), 40 d [(1.25 ± 0.24) vs (1.76 ± 0.40) cm3, P <0.05]. No significant antitumor effect was observed between 300 mg CTX combined HLI group and HLI alone group. None of the mice in the treatment group developed GVHD. The chimerism of mice in 80,200 mg CTX combined with HLI group was lower than 300 mg CTX combined with HLI group, and the disappearance time was earlier than the latter. CONCLUSION: Low dose CTX combined with irradiation of irradiated hemiphagic donor lymphocytes can obtain a better anti-tumor effect in mice with transplanted hepatocellular carcinoma. The antitumor effect of CTX is not enhanced after the CTX dose is increased.