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热激蛋白是生物体抵御逆境胁迫的重要蛋白,高温胁迫是诱导热激蛋白产生的重要因素,其中Hsp100蛋白可以在高温诱导时大量产生。本研究以草菇菌株V23和VH3为试验材料,首先观察了热激处理对低温胁迫后草菇菌丝恢复生长情况的影响,然后测定了热激处理再进行低温胁迫后草菇hsp100基因的表达量变化。研究结果显示:热激处理显著提高了草菇V23与VH3的恢复生长速度,增加了二者菌丝体中hsp100基因的表达量,有助于增强草菇对低温胁迫的耐受性。
Heat-shock protein is an important protein of living body to resist stress. High-temperature stress is an important factor to induce heat-shock protein production, in which Hsp100 protein can be produced in large quantities when induced by high temperature. In this study, the mushroom strains V23 and VH3 as the experimental material, first observed heat shock treatment of hyphal growth after mushroom mycelium growth and then heat shock treatment and then measured the straw mushroom hsp100 gene expression The amount of change. The results showed that heat shock treatment significantly increased the recovery rate of V23 and VH3 and increased the expression of hsp100 in both mycelia, which is helpful to enhance the tolerance of mushroom to low temperature stress.