本试验以具无融合生殖特性的4 年生湖北海棠实生树为试材, 以1 年生实生苗和4 年生成年芽嫁接树作为4 年生实生树的童区和成年区的对照。从空间角度对不同高度位置叶片的解剖结构进行研究。结果显示:多年生实生树叶片的解剖结构随叶位上升, 逐由阴性结构转变为阳性结构, 其叶片厚度、栅栏组织和海绵组织厚度, 细胞形状指数等均随叶片位置高度上升而增加。单位刻度内的栅栏组织细胞排列数却不随高度而发生变化, 且在成年芽嫁接树与实生树之间也无差异。成年芽嫁接树叶片的海绵组织厚度不随高度而发生变化, 但细胞形状指数、叶片和栅栏组织厚度等与多年生实生树具相同变化趋势, 也具有由阴性结构向阳性结构转变的特点。解剖结构特征不宜作阶段转变的指标。 In this study, 4-year-old Begonia tubulosa seedlings with no fusion reproductive traits were used as experimental materials. One-year-old seedlings and four-year-old grafted trees were used as control. From the perspective of space, the anatomical structures of leaves at different heights were studied. The results showed that the anatomical structure of perennial tree leaves increased with leaf position and changed from negative to positive. The leaf thickness, palisade and sponge thickness, and cell shape index increased with leaf height increasing. The number of palisade tissue cells per unit scale did not change with the height, and there was no difference between the adult buds and the tree. The thickness of spongy tissue of grafted adult leaves did not change with the height, but the cell shape index, the thickness of palisade and palisade tissue had the same trend with perennial tree, and also had the change from negative structure to positive structure. Anatomy of the structure should not be an indicator of phase change.