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目的探讨武汉市美沙酮门诊治疗者丙型肝炎病毒(HCV)及不同基因型感染状况。方法用酶标记免疫(ELISA)法检测抗-HCV抗体,在86份抗-HCV抗体阳性的血清标本中,分别提取HCV RNA,通过逆转录巢式聚合酶链反应(RT-nested PCR)扩增C基因的羧基至E1基因的氨基端长度为474 bp的片段,测定其核苷酸序列,与GenBank中已知的HCV序列进行系谱分析,确定HCV基因型。结果武汉市美沙酮门诊332名治疗者中抗-HCV IgG阳性率为94.3%;其中86份血清的HCV序列通过系谱显示6a型71例,占82.5%;3b 7例,占8.2%;1a 5例,占5.8%;1b 3例,占3.5%。结论武汉市美沙酮门诊入组的吸毒者HCV感染以6a型为优势株,其次为3b,吸毒者中HCV感染率较高,且基因亚型呈现多样性。
Objective To investigate the status of hepatitis C virus (HCV) and different genotypes in methadone outpatients in Wuhan. Methods Anti-HCV antibodies were detected by enzyme-linked immunosorbent assay (ELISA). HCV RNA was extracted from 86 serum samples positive for anti-HCV antibody and amplified by reverse transcriptase nested polymerase chain reaction (RT-nested PCR) C gene to the carboxyl terminal E1 gene of 474 bp fragment length, determination of its nucleotide sequence, and GenBank known HCV sequence pedigree analysis to determine the HCV genotype. Results The positive rate of anti-HCV IgG in the 332 methadone clinics in Wuhan was 94.3%. Among them, the HCV sequences of 86 serum samples showed type-6a 71 cases (82.5%), 3b 7 cases (8.2%), 1a 5 cases , Accounting for 5.8%; 1b 3 cases, accounting for 3.5%. Conclusions HCV infection in methadone outpatient group of Wuhan City is dominated by type 6a, followed by 3b. Among HCV drug users, the HCV infection rate is high and the genotype of the virus is diverse.