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以滇桂艾纳香为原料,经热水抽提、Sevag法除蛋白、醇沉、DEAE-纤维素柱层析、Sephadex G-200、Sepharose 6 F.F.凝胶色谱纯化得到纯品BRP;经HPGPC检测分析表明,BRP为均一多糖,分子量为3.3×104 Da;用UV、IR、HPLC、HPGPC、GC-MS、甲基化、NMR(1H NMR、13C NMR、HMQC、HMBC)等方法对BRP结构进行表征,结果表明,BRP仅由呋喃果糖残基组成,以→2)Fruf(1→(或→1)Fruf(→2)方式链接,推导其结构式为:β-D-Fruf-{2[→1)-β-D-Fruf(2→]n-1}-β-D-Fruf.
The pure BRP was purified by the extraction of hot water, Sevag deproteinization, alcohol precipitation, DEAE-cellulose column chromatography, Sephadex G-200 and Sepharose 6 FF gel chromatography. The purified product was purified by HPGPC The results showed that BRP was a homogeneous polysaccharide with a molecular weight of 3.3 × 104 Da. The structure of BRP was determined by UV, IR, HPLC, HPGPC, GC-MS, methylation, 1H NMR, 13C NMR, HMQC and HMBC The results showed that BRP consisted only of fructofuranose residues, and the structural formula was as follows: → 2) Fruf (1 → (or → 1) Fruf (→ 2) → 1) -β-D-Fruf (2 →] n-1} -β-D-Fruf.