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为研究虾青素对冷冻损伤的大黄鱼精子细胞的保护作用,探讨其内在机制,我们将大黄鱼精子稀释于含10%二甲基亚砜和各浓度虾青素的冷冻稀释液中,冷冻并解冻后,显微镜观察发现,1.43×10-4mol/L的虾青素使大黄鱼精子寿命、运动时间和激活率分别增加13.8%、36.0%和5.5%,接近于鲜精;单细胞凝胶电泳显示,核DNA受损程度明显减少,彗星拖尾变短;流式细胞术结果显示,质膜和线粒体明显受到保护,线粒体质量提高了19.27%(P<0.05)。通过双层类脂膜法测定不同浓度虾青素对H+的传递能力,发现2%的虾青素能较高效率地传递H+。结果表明适当浓度的虾青素能通过传递H+维持线粒体功能,从而保护冷冻损伤的大黄鱼精细胞。
In order to study the protective effect of astaxanthin on frozen-damaged large yellow croaker sperm cells and to explore its intrinsic mechanism, we diluted large-sized yellow croaker sperm in a frozen dilution containing 10% dimethylsulfoxide and various concentrations of astaxanthin, frozen Microscopically, astaxanthin (1.43 × 10-4 mol / L) increased the lifespan, exercise time and activation rate of big yellow croaker by 13.8%, 36.0% and 5.5%, respectively, Electrophoresis showed that the damage of nuclear DNA was significantly reduced and the trailing of the comet was shortened. The results of flow cytometry showed that the plasma membrane and mitochondria were obviously protected, and the mitochondrial mass increased by 19.27% (P <0.05). The transmission of H + by astaxanthin at different concentrations was determined by bilayer lipid membrane method. It was found that 2% astaxanthin can transfer H + efficiently. The results show that the appropriate concentration of astaxanthin can protect Cryptocaryon spermatophores by transferring H + to maintain mitochondrial function.