论文部分内容阅读
目的 :研究人胚胎脊髓提取液对鼠胚脊髓前角运动神经元的营养作用。方法 :用人胚胎制备脊髓提取液 ,其蛋白浓度为 2 5 0 μg/ml。脊髓运动神经元来源于E12～E15d的SD鼠胚。实验组加入提取液 ,不加提取液的为对照组。培养后进行各项活性指标的鉴定 ,用IBASRel2 0图像分析仪测量AgNORs染色的银染蛋白颗粒面积。结果 :实验组多角神经元数较对照组明显增多 (P <0 0 5 ) ;突起较粗且长形成致密网络 ,其突起平均长度和生长指数与对照组比较 ,差异有显著性 (P <0 0 1) ;实验组嗜银蛋白颗粒成团块状或为散在于细胞核内的小银染颗粒 ,银染色面积明显比对照组增大 (P <0 0 5 ) ;实验组AchE含量明显比对照组增加 (P <0 0 1)。结论 :人胚脊髓中存在有促进胚鼠脊髓前角运动神经元突起生长、存活的内源性物质 ,且此种物质具有促进脊髓前角运动神经元rRNA的转录功能。提示脊髓内源性物质具有运动性神经营养因子活性。
Objective: To study the nutritional effects of human embryonic spinal cord extract on motor neurons in the anterior horn of spinal cord in mouse embryos. Methods: Spinal cord extracts were prepared from human embryos with a protein concentration of 250 μg / ml. Spinal cord motor neurons derived from E12 ~ E15d SD mouse embryos. Experimental group added extract, without extract as control group. After incubation, the activity indexes were identified, and the area of AgNORs stained silver stained protein particles was measured by IBASRel20 image analyzer. Results: The number of polygonal neurons in the experimental group was significantly higher than that in the control group (P <0 05). The protuberances were thick and grew densely. The average length and growth index of the protuberances in the experimental group were significantly different from those in the control group (P <0.05). The content of AchE in the experimental group was significantly higher than that in the control group Group increased (P <0.01). CONCLUSION: There is an endogenous substance that promotes the growth and survival of neurons in the anterior horn of embryonic spinal cord in human embryo spinal cord, and this substance has the function of promoting rRNA transcription of anterior horn motor neurons of spinal cord. Tip spinal cord endogenous substances with motor neurotrophic factor activity.