目的 :探讨硫代修饰c fos反义寡脱氧核苷酸的潜在药理学价值及分子机制。方法 :建立焦虑的动物模型 ,将硫代修饰c fos反义寡脱氧核苷酸注入大鼠侧脑室 ;高架十字迷宫和空场实验评估大鼠焦虑行为 ,免疫组织化学技术检测Fos蛋白表达 ,计算机图像分析系统定量。结果 :c fos反义寡核苷酸减少了下丘脑室旁核部位的Fos免疫反应阳性神经元 ,抑制率 5 7.0 3%。并可以拮抗应激所至的焦虑相关行为 ,与diazepam效果类似而不影响中枢神经系统兴奋性。结论 :提示c fos是焦虑形成机制中不可或缺的环节 ,c fos硫代修饰反义寡核苷酸具有潜在的抗焦虑作用 Objective: To investigate the potential pharmacological value and molecular mechanism of thio-modified c fos antisense oligodeoxynucleotides. Methods: An animal model of anxiety was established. The thio-modified c fos antisense oligodeoxynucleotide was injected into the lateral ventricle of rats. An an-formus maze and empty field test were performed to evaluate the anxiety behavior of rats. Fos protein expression was detected by immunohistochemistry. Image Analysis System Quantitation. Results: c fos antisense oligodeoxynucleotides reduced the Fos immunoreactive neurons in paraventricular nucleus of hypothalamus with the inhibition rate of 5 7.03%. And can antagonize the stress-related anxiety-related behavior, and diazepam similar effect without affecting the central nervous system excitability. Conclusion: It is suggested that c fos is an indispensable link in the mechanism of anxiety formation. C fos thio-modified antisense oligonucleotides have a potential anti-anxiety effect