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了解博卡病毒(Human Bocavirus,HBoV)在我国儿童急性呼吸道疾病中的感染情况。采用PCR扩增的方法对2005年10月~2006年1月收集的72例急性呼吸道感染的住院儿童鼻咽抽吸物(nasopharyngeal aspirates,NPA)进行了HBoV基因检测。将PCR阳性产物进行TA克隆,测序,并将所测序列与GenBank中HBoV序列进行比较分析。72份标本中共检测到6份HBoV阳性扩增产物,阳性率为8.3%(6/72),该6例HBoV阳性患儿临床均有肺炎或支气管肺炎症状。由此可以初步看出HBoV可能也是儿童急性呼吸道感染中较为重要的一个病原,且可能与儿童急性下呼吸道感染存在相关性。
To understand the infection of Bocavirus (HBoV) in children with acute respiratory diseases in our country. The HBoV gene was detected in 72 nasopharyngeal aspirates (NPA) hospitalized children with acute respiratory infection collected from October 2005 to January 2006 by PCR amplification. The PCR positive products were cloned and sequenced. The sequences were compared with HBoV sequences in GenBank. A total of 6 HBoV positive amplification products were detected in 72 samples, the positive rate was 8.3% (6/72). All 6 HBoV positive children had pneumonia or bronchopneumonia symptoms. It can be initially concluded that HBoV may also be a more important pathogen of childhood acute respiratory infection and may be related to acute lower respiratory tract infection in children.