目的 本文的目的是测定RhD基因是否在妊娠的任何阶段都能在滋养层里得到表达。研究方法 从18位妊娠者(妊娠8～40周)得到的滋养层和胎儿组织,从滋养层细胞提取DNA和RNA。使用RhD基因特异引物进行逆转录聚合酶链反应,从RNA里合成cDNA。从胎儿组织提取的DNA通过聚合酶链反应去测定胎儿的RhD状态,也可通过使用单克隆抗D抗体,通 Purpose The purpose of this paper is to determine if the RhD gene is expressed in the trophoblast at any stage of pregnancy. METHODS Taken from trophoblast and fetal tissue obtained from 18 pregnancies (8 to 40 weeks gestation), DNA and RNA were extracted from trophoblast cells. Reverse transcription polymerase chain reaction (RT-PCR) using RhD gene-specific primers to synthesize cDNA from RNA. DNA extracted from fetal tissue by polymerase chain reaction to determine the status of the fetus RhD, but also by using monoclonal anti-D antibodies, through