目的了解我国乙型肝炎病毒(HBV)C区变异的生物学意义.方法采用酶切位点消除法定点突变构建HBV C基因变异株(V60、G87、L97)真核细胞表达载体;转染HepG2细胞用ELISA法检测各毒株宿主细胞上清液中HBeAg.结果与野生毒株相比,构建的变异株前C/C区碱基除目的突变点外,其他碱基均同源.在重组质粒转染的宿主细胞中检测到目的DNA片段.L97变异株宿主细胞上清液HBeAg的A值在不同时
Background Data on the epidemiology of hypertension in Chinese non-dialysis chronic kidney disease (CKD) patients are limited.The aim of the present study was t
群体性氨气中毒是较为常见的突发公共卫生事件,具有中毒人数多、病情变化快、救治困难等特点。我院从2013年5月至2015年9月收治98例氨气吸入性损伤患者,现就其院前急救的意义
Background Human piebaldism is a rare autosomal dominant condition characterized by congenital white forelock and depigmented patches of skin,typically on the f
在目前建筑工程基础设计当中,随着高层建筑工程越来越多,围绕现高层建筑设计当中对于地基基础如未深入研究情况下,基础结构形式设计过分保守,这会给项目投资和资源造成浪费。
根据某旅游公路采用同步碎石封层进行路面预防性养护的工程实例,对施工后出现的纵向条纹和表面松散和剥落等问题进行了深入分析,探讨了其产生的原因所在,同时,指出了同步碎石
Background Occlusal splints have been the preferred modalities in the management of myofascial temporomandibular disorders (TMDs),but now controversy exists in
Background Although neuroradiological findings of M(o)bius syndrome have been reported as a result of brain and brainstem abnormalities,magnetic resonance imagi