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目的 :观察哮喘和过敏性肺炎 ( HP)患者肺泡巨噬细胞 ( AM)释放白细胞介素 - 12 ( IL - 12 )能力的差异并探讨其意义。方法 :对哮喘及 PH患者各 10例进行支气管肺泡灌洗 ( BAL)获取 AM,经脂多糖刺激培养后取上清液用双抗体夹心酶联免疫吸附法 ( EL ISA)测量 IL- 12含量 ,并与 10例健康者作对照。结果 :哮喘组 IL- 12水平不但低于 HP组也低于对照组( P均 <0 .0 1) ;HP组 IL - 12水平不但高于哮喘组也高于对照组 ( P <0 .0 1)。结论 :IL - 12水平的改变在哮喘及 HP二者的病理过程中起着重要作用
Objective: To investigate the difference of the ability of alveolar macrophages (AM) releasing interleukin - 12 (IL - 12) in asthmatic patients and allergic pneumonia (HP). Methods: AM was obtained by bronchoalveolar lavage (BAL) in 10 cases of asthmatic and PH patients. After stimulated by lipopolysaccharide, the supernatant was collected and the content of IL-12 was measured by double antibody sandwich enzyme-linked immunosorbent assay (ELISA) And compared with 10 healthy people. Results: The level of IL - 12 in the asthma group was lower than that in the HP group (P <0.01). The level of IL - 12 in the HP group was higher than that in the asthma group (P <0. 1). Conclusions: Changes in IL - 12 levels play an important role in the pathogenesis of both asthma and HP