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目的研究小鼠UNCV蛋白质对HeLa细胞凋亡的影响。方法将BALB/c小鼠Uncv基因重组质粒转染HeLa细胞,筛选出稳定过表达UNCV蛋白质的HeLa细胞株。通过细胞计数法和流式细胞术,检测过表达UNCV蛋白质对血清饥饿和阿霉素诱导的HeLa细胞凋亡的影响。结果获得稳定正确过表达UNCV蛋白质的HeLa细胞株。细胞计数和流式细胞术结果显示过表达UNCV蛋白质对血清饥饿诱导的HeLa细胞凋亡有抑制作用,对于阿霉素诱导的HeLa细胞凋亡没有显著影响。结论过表达UNCV蛋白质对血清饥饿诱导的HeLa细胞凋亡有抑制作用。
Objective To study the effect of mouse UNCV protein on the apoptosis of HeLa cells. Methods HeLa cells were transfected with Uncv recombinant plasmid of BALB / c mice and HeLa cell lines stably expressing UNCV protein were screened out. The effect of over-expressed UNCV protein on serum starvation and doxorubicin-induced apoptosis of HeLa cells was examined by cytometry and flow cytometry. Results HeLa cell lines stably and correctly over-expressing UNCV protein were obtained. The results of cell counting and flow cytometry showed that over-expression of UNCV protein could inhibit the apoptosis of HeLa cells induced by serum starvation and had no significant effect on doxorubicin-induced HeLa cell apoptosis. Conclusion Over-expression of UNCV protein can inhibit the apoptosis of HeLa cells induced by serum starvation.