论文部分内容阅读
为进一步探索正常Ｐ５３ 对肿瘤细胞增殖的抑制作用，本文用磷酸钙ＤＮＡ共沉淀法，将本室构建的ＰＸＴ１Ｐ５３ 真核表达细胞转移至ＨｅｐＧ２ 肝癌细胞系内，经斑点杂交技术和间接免疫荧光技术证实，外源性Ｐ５３ 基因已在ＨｅｐＧ２ 细胞中稳定表达，导入的Ｐ５３基因亦能抑制ＨｅｐＧ２ 肝癌细胞系增殖并诱导其凋亡。实验结果提示正常Ｐ５３ 基因可成为治疗不同肿瘤的重要靶分子之一。
In order to further explore the normal P53 inhibition of tumor cell proliferation, this paper with calcium phosphate co-precipitation method, the room constructed PXT1 P53 eukaryotic cells transfected HepG 2 hepatoma cell line, by dot blot hybridization and Indirect immunofluorescence confirmed that exogenous P53 gene has been stably expressed in HepG 2 cells, the introduction of P53 gene also inhibited HepG 2 hepatoma cell line proliferation and induce apoptosis. The experimental results suggest that the normal P53 gene may be one of the important target molecules for treating different tumors.