论文部分内容阅读
目的研究慢性血吸虫病患者对乙型肝炎(乙肝)疫苗保护性免疫的影响。方法随机筛选无乙肝病毒感染的慢性血吸虫病患者(实验组)24例,健康志愿者(对照组)26例。分别收集实验组和对照组接种乙肝疫苗前后血清,用ELISA法检测两组接种前后血清乙肝病毒表面抗体(抗-HBs)、肿瘤坏死因子-α(TNF-α)及白细胞介素-2(IL-2)的水平。结果接种乙肝疫苗前实验组和对照组抗-HBs平均吸光度(A值)分别为0.134和0.150。接种后,实验组血清抗-Hbs平均A值为0.217,转阳率为17%,与接种前相比差异无统计学意义(P>0.05);对照组抗-Hbs平均A值为1.210,转阳率为92%,与接种前相比差异有显著的统计学意义(P<0.01)。实验组接种乙肝疫苗前血清IL-2和TNF-α平均水平均明显高于对照组,接种后两组血清IL-2和TNF-α平均水平均升高,但与接种前相比差异无统计学意义(P>0.05)。结论慢性血吸虫病患者对乙肝疫苗的免疫应答水平低下。
Objective To study the influence of chronic schistosomiasis on protective immunity of hepatitis B (HBV) vaccine. Methods 24 patients with chronic schistosomiasis without hepatitis B virus infection (experimental group) and 26 healthy volunteers (control group) were randomly selected. The serum levels of hepatitis B virus (HBsAg), tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2) were measured by ELISA before and after inoculation of experimental group and control group -2) level. Results Before inoculation of hepatitis B vaccine, the average absorbance (A value) of anti-HBs in experimental group and control group were 0.134 and 0.150, respectively. After inoculation, the serum anti-Hbs in the experimental group had a mean A value of 0.217 and a positive rate of 17%, which showed no significant difference compared with that before inoculation (P> 0.05). The average A value of anti-Hbs in the control group was 1.210 The positive rate was 92%, which was significantly different from that before inoculation (P <0.01). The mean levels of serum IL-2 and TNF-α in experimental group were significantly higher than those in control group before inoculation of hepatitis B vaccine. The average level of IL-2 and TNF-α in serum of two groups increased after inoculation, but there was no statistical difference Significance (P> 0.05). Conclusions Chronic schistosomiasis patients have low level of immune response to hepatitis B vaccine.