论文部分内容阅读
本研究利用大鼠肝膜泌乳素受体为特异竞争结合蛋白，在国内首次建立了泌乳素放射受体分析法。该方法对泌乳素测定具有高度特异性，灵敏度为５．０±１．４ｍＩＵ／ｄｌ，批内和批间变异系数分别不超过８．８％和１１．３％，泌乳素的血清回收率为９８．６±５．０％。对垂体腺瘤的研究发现，血清泌乳素至少存在三种不同的分子形式。其表现分子量分别为９８．６ｋＤａ、５０．９ｋＤａ和２４．６ｋＤａ，它们的受体结合活性分别为５．４２ＩＵ／ｍｇ、１１．２２ＩＵ／ｍｇ和３９．６０ＩＵ／ｍｇ。与垂体非泌乳素腺瘤相比，在垂体泌乳素腺瘤患者血清中“巨”泌乳素和“大”泌乳素所占比例较高，而“小”泌乳素显著降低。
In this study, rat hepatic prolactin receptor as a specific competitive binding protein, the first time in China established prolactin receptor analysis. The method has a high specificity for prolactin determination with a sensitivity of 5.0 ± 1.4 mIU / dl, intra-assay and inter-assay coefficients of variation of no more than 8.8% and 11.3%, respectively, and prolactin serum recovery of 98.6 ± 5.0%. The study of pituitary adenoma found that serum prolactin at least three different molecular forms. Their expressed molecular weights were 98.6 kDa, 50.9 kDa and 24.6 kDa, respectively, and their receptor binding activities were 5.42 IU / mg, 11.22 IU / mg and 39.60 IU / mg, respectively. In contrast to pituitary non-prolactinomas, the proportion of “giant” prolactin and “large” prolactin in the serum of patients with pituitary prolactinomas is significantly higher, while the “small” prolactin is significantly reduced.