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目的观察C57BL/6J小鼠老年性听力下降现象并研究中药复方健耳剂对老年性聋小鼠的保护作用及其可能机制。方法将36只C57BL/6J小鼠分为正常对照组(n=6)、老年性聋对照组(n=12)及高剂量中药健耳剂组(简称中药高剂量组,n=12)及低剂量中药健耳剂组(简称中药低剂量组,n=6)。正常对照组小鼠自断奶后每日饮用自来水直至出生后2个月;老年性聋对照组小鼠自断奶后每日饮用自来水直至出生后7个月;中药高、低剂量组小鼠自断奶后分别每日饮用3.65、0.91 g/(kg·d)的中药复方健耳剂直至出生后7个月。每组6只小鼠在实验终止日应用听性脑干诱发电位仪检测小鼠的听性脑干反应(auditory brainstem response,ABR)阈。中药高剂量组与同期老年性聋对照组6只小鼠在终止实验即刻取出耳蜗、听皮层脑组织及肝脏,采用丙二醛考马斯亮蓝试剂盒,利用紫外分光光度计,检测所取组织中丙二醛含量。结果2月龄正常对照组小鼠在4~48 KHz频率范围的不同短纯音诱发的ABR阈值保持正常。与2月龄正常对照组比较,7月龄老年性聋对照组小鼠ABR阈值明显升高(P<0.05),与高剂量中药组在8~48 KHz频率范围ABR阈值比较,差异亦有统计学意义(P<0.05)。与7月龄老年性聋对照组比较,高剂量中药组小鼠耳蜗、听皮层、肝脏组织中丙二醛含量明显降低(P<0.01)。结论 C57BL/6J小鼠在出生后7个月表现出明显的高频性老年性聋症状。每日饮用高剂量中药健耳剂可显著延缓老年性聋的发生和发展。中药健耳剂保护听觉延缓老年性聋发生的作用,可能与其所含中药的抗氧化效应有关。
Objective To observe the senile dementia in C57BL / 6J mice and to study the protective effect and possible mechanism of traditional Chinese medicine compound auris in the senile deaf mice. Methods 36 C57BL / 6J mice were divided into normal control group (n = 6), senile deafness control group (n = 12) and high dose of traditional Chinese medicine (group A, n = 12) Low dose of traditional Chinese medicine and fungus group (referred to as low-dose group of Chinese medicine, n = 6). Normal control mice were fed daily with tap water after weaning until 2 months after birth. Mice with senile deafness drank tap water daily for 7 months after weaning, and mice in high and low dose groups were self-weaning After daily drinking 3.65,0.91 g / (kg · d) of Chinese herbal compound auris into 7 months after birth. The auditory brainstem response (ABR) threshold of 6 mice in each group was measured by auditory brainstem response potentiometry at the termination of the experiment. Six rats from the high-dose group and the senile deafness control group were immediately removed from the cochlea, auditory cortex brain and liver in the termination experiment. Malondialdehyde-Coomassie brilliant blue kit was used to detect the content of Malondialdehyde content. RESULTS: The ABR thresholds induced by different short pure tones in 2-month-old normal control mice remained normal in the frequency range of 4-48 KHz. Compared with the 2-month-old normal control group, the ABR threshold in the 7-month-old aged deafness control group was significantly increased (P <0.05). Compared with the high dose traditional Chinese medicine group, the ABR threshold in the frequency range of 8 ~ 48 KHz was also statistically different Significance (P <0.05). Compared with the control group of 7-month-old senile deafness, the content of malondialdehyde in cochlear, auditory cortex and liver of high-dose traditional Chinese medicine group was significantly decreased (P <0.01). Conclusion C57BL / 6J mice showed obvious symptoms of senile deafness at 7 months after birth. Daily consumption of high doses of traditional Chinese medicine and auris can significantly delay the occurrence and development of senile deafness. The effect of traditional Chinese medicine for the protection of auditory delaying senile deafness may be related to the anti-oxidant effect of traditional Chinese medicine.