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【目的】研究外源物质处理对低温胁迫过程中辣椒幼苗生理生化的影响。【方法】用0.5 mmol/L外源亚精胺、50 mg/L壳聚糖、2 mmol/L水杨酸和10 mmol/L甜菜碱喷施处理辣椒幼苗,并将幼苗置于昼温/夜温为10℃/5℃条件下进行低温胁迫处理,分别在胁迫0、2、4、6和8 d,25℃/20℃恢复2 d后取样测定可溶性糖、脯氨酸、可溶性蛋白、丙二醛含量和SOD、POD活性、叶绿素含量等生理指标,探讨外源物质对低温胁迫过程中辣椒幼苗生理活动的影响。【结果】适宜浓度的外源亚精胺、壳聚糖、水杨酸、甜菜碱处理能明显地降低低温胁迫过程中辣椒幼苗叶片中的MDA含量,增加可溶性糖、脯氨酸、可溶性蛋白含量和叶绿素含量,增强SOD和POD活性。【结论】4种外源物质均可不同程度地提高辣椒幼苗的抗冷性,作用效果最好的物质依次为亚精胺、壳聚糖、甜菜碱、水杨酸。
【Objective】 The objective of this study was to investigate the effects of exogenous substances on physiological and biochemical characteristics of pepper seedlings under chilling stress. 【Method】 The pepper seedlings were sprayed with 0.5 mmol / L exogenous spermidine, 50 mg / L chitosan, 2 mmol / L salicylic acid and 10 mmol / L betaine, and the seedlings were placed in daytime temperature / Under night temperature of 10 ℃ / 5 ℃, low temperature stress treatment was carried out. Soluble sugar, proline, soluble protein, soluble protein and soluble protein were detected at 0, 2, 4, 6 and 8 d after stress and 25 ℃ / MDA content, SOD activity and POD activity, chlorophyll content and other physiological indexes to investigate the effects of exogenous substances on the physiological activities of pepper seedlings under chilling stress. 【Result】 Appropriate concentrations of exogenous spermidine, chitosan, salicylic acid and betaine significantly reduced the content of MDA and increased the contents of soluble sugar, proline and soluble protein in pepper seedling leaves during cold stress And chlorophyll content, increase SOD and POD activity. 【Conclusion】 All four exogenous substances can improve chilling tolerance of pepper seedlings to varying degrees. The most effective substances were spermidine, chitosan, betaine and salicylic acid.