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该研究以在樟子松凋落物层中高频出现的3株丝状真菌Alternaria sp.、Penicillium sp.和Pestalotiopsis sp.为供试菌株,以樟子松新鲜落叶为作用底物,通过发酵纯培养的方法,测定了底物有机物质质量损失及发酵过程中产生的漆酶(Laccase)、锰过氧化物酶(MnP)、羧甲基纤维素酶(CMCase)和滤纸酶(FPA)酶活性变化,并验证了酶活性与底物降解的关系。结果表明,Alternaria sp.引起底物总有机物质质量损失最大,且产生的漆酶、羧甲基纤维素酶和滤纸酶活性都较高;Peni-cillium sp.产生的锰过氧化物酶活性最高。3株丝状真菌同属于真菌功能群中的木质纤维素分解者。
In this study, three filamentous fungi Alternaria sp., Penicillium sp. And Pestalotiopsis sp., Which appeared in the litter of Pinus sylvestris var. Mongolica at high frequency, were used as test strains. Fresh leaves of Pinus sylvestris were used as substrates. , The changes of the mass loss of organic substances and the enzyme activities of laccase, manganese peroxidase (MnP), carboxymethylcellulase (CMCase) and filter paper enzyme (FPA) produced during fermentation were determined. , And verified the relationship between enzyme activity and substrate degradation. The results showed that Alternaria sp. Caused the highest mass loss of total organic matter, and produced higher activity of laccase, carboxymethyl cellulose and filter paper enzymes. Peni-cillium sp. Produced the highest manganese peroxidase activity . The three filamentous fungi belong to the same group of lignocellulose decomposers in fungal functional groups.