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目的:探讨喉鳞状细胞癌组织中Ⅰ型胶原(ColⅠ)、Ⅳ型胶原(ColⅣ)、纤维连接蛋白(Fn)的表达及其与肿瘤侵袭和转移的关系。方法:应用免疫组织化学方法检测60例喉鳞状细胞癌患者的癌旁正常组织和癌组织标本中ColⅠ、ColⅣ、Fn的表达,应用VIDAS图像分析仪分析其图像的积分光吸收度(iOD),并进行统计学处理。结果:在癌周正常组织中,ColⅠ表达明显且完整,ColⅣ和基膜Fn表达呈现出一种完整的线状结构,间质Fn则呈完整的网状结构;而在鳞状细胞癌组织中,随分化程度降低,三者的表达逐渐下降,完整性亦逐渐破坏和消失,其变化的差异有统计学意义(P<0.05或P<0.01)。喉鳞状细胞癌组织中ColⅠ、ColⅣ、Fn的表达表现出高度一致性,随着肿瘤体积的增大、病理分级和临床分期的进展、颈淋巴结的转移,其表达均逐渐下降,且表达的变化差异具有统计学意义(P<0.05或P<0.01)。结论:喉鳞状细胞癌组织中ColⅠ、ColⅣ、Fn的表达与肿瘤侵袭和转移等生物学特性密切相关,对了解喉鳞状细胞癌区域性淋巴结转移的可能性、手术方法的选择及预后判断具有一定的意义。
Objective: To investigate the expression of Col Ⅰ, Col Ⅳ and Fn in laryngeal squamous cell carcinoma and its relationship with tumor invasion and metastasis. Methods: Immunohistochemical method was used to detect the expression of ColⅠ, ColⅣ and Fn in 60 normal laryngeal squamous cell carcinoma tissues and adjacent normal tissues. VIDAS image analyzer was used to analyze the integral optical absorption (iOD) , And statistical processing. Results: In normal peritumoral tissues, the expression of ColⅠ was obvious and complete. The expression of Col Ⅳ and Fn in basement membrane showed a complete linear structure, while the interstitial Fn showed a complete network. In squamous cell carcinoma , With the degree of differentiation decreased, the expression of the three gradually decreased, the integrity also gradually destroyed and disappeared, the difference was statistically significant (P <0.05 or P <0.01). The expression of ColⅠ, ColⅣ, Fn in laryngeal squamous cell carcinoma showed a high degree of consistency. With the increase of tumor volume, the progress of pathological grade and clinical stage, the metastasis of cervical lymph node, the expression of ColⅠ, The difference was statistically significant (P <0.05 or P <0.01). CONCLUSION: The expressions of ColⅠ, ColⅣ and Fn in laryngeal squamous cell carcinoma are closely related to the biological characteristics such as tumor invasion and metastasis. To understand the possibility of regional lymph node metastasis of laryngeal squamous cell carcinoma, the choice of surgical method and prognosis Has a certain meaning.