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研究人生长激素 (h GH)对体外培养的结肠癌细胞株增殖周期和 DNA倍体的影响和意义。取指数生长期的结肠癌细胞株 L S- 174- T(简称 174) ,以顺铂和 (或 )不同浓度的 h GH体外培养 ,2 4h后以流式细胞仪测定细胞增殖周期等指标。结果 :h GH能诱导 174细胞 S期数率显著上升 (P0 .
To study the effect and significance of human growth hormone (hGH) on the proliferation cycle and DNA ploidy of colon cancer cell lines cultured in vitro. Colon cancer cell line L S-174-T (abbreviated as 174) was taken from exponential growth phase and cultured in vitro with cisplatin and / or h GH at different concentrations. Cell proliferation cycles were measured by flow cytometry after 24 hours. Results: h-GH induced a significant increase in S phase of 174 cells (P0.