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目的:建立高效液相色谱法测定穿龙骨刺片中淫羊藿苷的含量。方法:Agilent C_(18)色谱柱(250 mm×4.6 mm,5μm),0.075 mo·L~(-1)磷酸溶液(用三乙胺调pH=4.5)-乙腈(75:25)为流动相,流速0.8ml·min~(-1)检测波长270 nm。结果:淫羊藿苷在进样量0.02～0.16μg范围内,与峰面积呈良好的线性关系(r=0.999 9),平均回收率为99.2%(rSD=1.0%,n= 9)。结论:方法准确可靠、重复性好,简便快速,可用于该制剂的质量控制。
Objective: To establish a HPLC method for the determination of icariin content in Tonglong Gugu Ci tablet. Method: Agilent C 18 column (250 mm × 4.6 mm, 5 μm), 0.075 mo·L -1 phosphoric acid solution (pH adjusted to 4.5 with triethylamine)-acetonitrile (75:25) as mobile phase The flow rate was 0.8ml·min~(-1) and the detection wavelength was 270 nm. Results: In the range of 0.02-0.16 μg injection volume, icariin showed a good linear relationship with the peak area (r=0.999 9). The average recovery was 99.2% (rSD=1.0%, n=9). Conclusion: The method is accurate, reliable, reproducible, simple and rapid, and can be used for the quality control of the preparation.