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以抗寒力不同的毛果绣线菊与金山绣线菊为试验材料,测定低温下光合特性的变化,利用蛋白质组学核心技术建立两种绣线菊低温下光合作用蛋白的表达图谱,并鉴定光合作用蛋白种类,探讨两种绣线菊对低温锻炼的响应机制。结果表明:低温下两种绣线菊的光合能力下降,利用弱光的能力增强,利用强光的能力降低,抗寒能力较强的毛果绣线菊利用弱光能力强于抗寒能力较弱的金山绣线菊。在不同低温水平下分别鉴定出涉及能量代谢与运输的光合作用蛋白共17种,低温下金山绣线菊中参与卡尔文循环、三羧酸循环、磷酸戊糖途径和光合磷酸化的酶显著上调和下调表达,毛果绣线菊中仅参与卡尔文循环的酶和参与PSⅡ光合电子传递的蛋白明显上调和下调表达;低温使两种绣线菊光合作用均受到影响,金山绣线菊光合作用蛋白在低温下的变化更为显著。
Cold-tolerant Coprinus comatus and Jinshan Spiraea indica were used as materials to determine the changes of photosynthetic characteristics at low temperature. The expression patterns of photosynthetic proteins at two temperatures of Spiraea polyphylla were established by using proteomics core technologies. Identify the types of photosynthetic proteins and explore the response mechanisms of two species of Spiraea to low temperature exercise. The results showed that under low temperature, the photosynthetic capacity of two species of Spiraea decreased, the ability to use weak light was enhanced, the ability to use light was reduced, and the cold hardiness ability of Spiraeda fasciatus was stronger than that of cold hardiness Weak Jinshan meadowsweet. A total of 17 photosynthetic proteins involved in energy metabolism and transport were identified at different hypothermia levels. The enzymes involved in calvin cycle, tricarboxylic acid cycle, pentose phosphate pathway and photosynthetic phosphorylation were significantly up-regulated And down-regulated expression. The enzymes involved in Calvin cycle and the proteins involved in photosynthetic electron transport of PSⅡwere significantly up-regulated and down-regulated in the fruit of Spiraea polycarpna. The photosynthesis of the two species of Spiraea intermedia was affected by the low temperature. Protein changes at low temperatures are more pronounced.