论文部分内容阅读
[目的]研究β2肾上腺素能受体基因(β2-adrenergic receptor,β2-AR)Arg16/Gly多态性与哈萨克族人超重、肥胖的相关性。[方法]随机入选557例新疆北部的哈萨克族牧民,男性232例,女性325例,年龄30～70岁,按体重指数(BMI)﹤24kg/m2、24～27.9kg/m2、≥28kg/m2将入选人群划分为正常、超重及肥胖组3组。用聚合酶链反应-限制性酶切片段多态性(PCR-RFLP)技术分析入选人群的β2-AR基因Arg16/Gly多态性,观察各基因型和等位基因在正常、超重及肥胖3组中的分布频率及其与超重、肥胖的关系。[结果]正常、超重、肥胖3组间相应Arg16/Arg、Arg16/Gly、Gly16/Gly3种基因型频率分别为0.28、0.45、0.27,0.36、0.46、0.18和0.33、0.43、0.24;3组间Arg16、Gly16等位基因频率分别为0.51、0.49,0.59、0.41和0.55、0.45,3种基因型及二种等位基因在正常、超重及肥胖3组中的分布频率差异均无统计学意义(P﹥0.05)。分别比较3种基因型在哈萨克族男性、女性人群中正常、超重及肥胖3组中的分布频率也未发现统计学差异。[结论]哈萨克族人群β2-AR基因Arg16/Gly多态性与哈萨克族人群的超重、肥胖无相关性。
[Objective] To investigate the correlation between the Arg16 / Gly polymorphism of β2-adrenergic receptor (β2-AR) and Kazakh overweight and obesity. [Methods] A total of 557 Kazak herders in northern Xinjiang were enrolled. There were 232 males and 325 females, aged 30-70 years old. According to the body mass index (BMI) <24kg / m2, 24-27.9kg / m2, The selected population was divided into three groups: normal, overweight and obesity group. The polymorphism of Arg16 / Gly of β2-AR gene in the selected population was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and the genotypes and alleles were observed in normal, overweight and obese 3 Distribution frequency in the group and its relationship with overweight and obesity. [Results] The genotype frequencies of Arg16 / Arg, Arg16 / Gly and Gly16 / Gly in normal, overweight and obese groups were 0.28, 0.45, 0.27, 0.36, 0.46, 0.18 and 0.33, 0.43 and 0.24 respectively. Arg16, Gly16 allele frequencies were 0.51,0.49,0.59,0.41 and 0.55,0.45 three genotypes and two alleles in the normal, overweight and obese three groups the distribution frequency was not statistically significant ( P> 0.05). No statistical difference was found in the distribution frequency of the three genotypes among the normal, overweight and obese groups in Kazak men and women. [Conclusion] There is no correlation between Arg16 / Gly polymorphism of β2-AR gene and Kazakh overweight and obesity in Kazak population.