Objective: To determine the larvicidal activities of petroleum ether, chloroform, ethyl acetate and methanol fractions of roots and fruits extracts of Astrodaucus persicus from Apiaceae family against malaria vector, Anopheles stephensi(An. stephensi).Methods: Twenty five third instar larvae of An. stephensi were exposed to various concentrations(10–160 g/L) of fractions and were assayed according to World Health Organization protocol. The larval mortality was calculated after 24 h treatment.Results: Among tested fractions, the highest larvicidal efficacy was observed from ethyl acetate fraction of fruits extract with 50% and 90% mortality values(LC_(50) and LC_(90)) of34.49 g/L and 108.61 g/L, respectively. Chloroform fraction of fruits extract was the second larvicidal sample with LC_(50) of 45.11 g/L and LC_(90) of 139.36 g/L. Petroleum ether fractions of fruits and roots and methanol fraction of fruits showed moderate toxicity against An. stephensi.Conclusions: Astrodaucus persicus is a potential source of valuable and natural larvicidal compounds against malaria vector, An. stephensi and can be used in mosquitoes control programs as an alternative to synthetic insecticides. Objective: To determine the larvicidal activities of petroleum ether, chloroform, ethyl acetate and methanol fractions of roots and fruits extracts of Astrodaucus persicus from Apiaceae family against malaria vector, Anopheles stephensi (An. Stephensi). Methods: Twenty five third instar larvae of An . The larval mortality was calculated after 24 h treatment. Results: Among the tested fractions, the highest larvicidal efficacy was observed from ethyl acetate fraction of fruits extract with 50% and 90% mortality values ​​(LC_ (50) and LC_ (90)) of 34.49 g / L and 108.61 g / L, respectively. Chloroform fraction of fruits extract was the second larvicidal sample with LC_ (50) of 45.11 g / L and LC_ (90) of 139.36 g / L. Petroleum ether fractions of fruits and roots and methanol fraction of fruits showed moderate toxicity against An. Stephensi. Conclusions: Astrodaucus persic us is a potential source of valuable and natural larvicidal compounds against malaria vector, An. stephensi and can be used in mosquitoes control programs as an alternative to synthetic insecticides.