论文部分内容阅读
患儿,女,7岁,右眼睑周围皮肤红斑丘疹伴瘙痒2周,外用糖皮质激素药膏后加重,皮疹扩大。取患者皮疹边缘皮屑行真菌学检查,直接镜检可见透明有隔真菌菌丝,真菌培养结果鉴定为须癣毛癣菌生长;取患儿家庭所饲养宠物兔耳部皮损皮屑及毛发行真菌学检查,直接镜检可见发内链状孢子,真菌培养结果为须癣毛癣菌。提取病原菌DNA,利用引物ITS4和ITS5进行r DNA ITS区序列的PCR扩增,扩增产物测序后在Genbank核酸数据库中进行同源性比对。证实患者面癣病原菌与家兔被毛所携带的须癣毛癣菌同源性达99%。患者经抗真菌治疗后痊愈。
Children, women, 7 years old, erythema rash associated with pruritus around the right eyelid for 2 weeks, topical glucocorticoid cream exacerbated, rash enlarged. Take patients with rash edge dandruff mycological examination, the direct microscopic examination can be seen with transparent fungal mycelium, fungal culture was identified as fungus Trichophyton mentagrophytes growth; take the family of pet rabbits ear skin lesions dander and hair Distribution of mycology check, direct microscopic examination of the hair inside the chain spores, fungal culture results for Trichophyton mentagrophytes. The DNA of the pathogen was extracted and the ITS region of the rDNA was amplified by using the primers ITS4 and ITS5. The amplified products were sequenced and aligned in the GenBank database. Confirmed patients with ringworm pathogens and rabbit coat Trichophyton mentagrophytes carry 99% homology. The patient recovered after anti-fungal treatment.