1.双注射的标志方法双注射法是由两次单独的注射组成的,首先进行初级染色,然后进行次级染色。按前面所述的方法将初级染料的水溶液——固绿或台盼兰注射入实验对虾体内,次级染色物质用1或2ml结核菌素注射器25或27号针头注射。0.003～0.01ml的次级染色物质注射到中部背 1. Dual Injection Marking Method The dual injection method consists of two separate injections, with primary staining followed by secondary staining. Injection of green dye or trypan blue into the body of experimental shrimp was performed as previously described, with secondary dyeings injected with 1 or 2 ml of tuberculin syringe 25 or 27 gauge needle. 0.003 ~ 0.01ml of secondary staining material injected into the middle back