论文部分内容阅读
目的研究生活饮用水中损伤性非苛养菌的耐药性。方法采用增菌培养基和选择性培养基对某小区生活饮用水进行损伤菌分离,获得分离株后,利用16S rRNA基因测序法进行种属鉴定;采用Kirby-Bauer纸片扩散法进行细菌的抗菌药物敏感性试验。结果在分离的58株损伤性非苛养菌中,耐药菌占51.72%,包括肠球菌属、肠杆菌属、葡萄球菌属、不动杆菌属、假单胞菌属。其中80%的损伤菌表现出对两种及以上抗菌药物耐药。耐药菌对复方新诺明的耐药最为普遍,耐药率为63.33%;对磺胺甲噁唑的耐药次之,占43.33%。结论测试小区生活饮用水中的损伤性非苛养菌耐药性较为普遍,应加强关注其潜在的人群健康风险。
Aim To study the drug resistance of non-fastidious bacteria in drinking water. Methods Isolates were isolated from the drinking water of living quarters by enrichment medium and selective medium. After isolates were obtained, the 16S rRNA gene sequencing was used to identify the species. Kirby-Bauer disk diffusion method was used to detect the antibacterial activity of the bacteria Drug sensitivity test. Results Among the 58 isolates of non-nociceptive bacteria isolated, the resistant strains accounted for 51.72%, including Enterococcus, Enterobacter, Staphylococcus, Acinetobacter and Pseudomonas. About 80% of them show resistance to two or more antibiotics. Drug-resistant strains of cotrimoxazole resistance is the most common, resistance rate was 63.33%; resistance to sulfamethoxazole followed, accounting for 43.33%. Conclusion The resistance of non-nocturnal non-fastidious bacteria in the drinking water of test plot is relatively common, and the health risk of potential population should be paid more attention.