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探索从微量植物检材中提取DNA的有效方法,并尝试应用于法庭科学实践。方法:采用优化改良的硅珠法,从10种不同的植物叶子中提取DNA(8种常见植物,2种毒品原植物)。通过紫外分光光度法、琼脂糖凝胶电泳、PCR扩增及限制性内切酶消化对提取的DNA进行检测;最后以实际案例中的植物检材为研究对象,验证提取方法。结果:通过该方法提取的植物DNA纯度较高,质量较好。PCR扩增的条带清晰、明亮,无杂带。结论:该方法可有效去除次生物质对DNA的干扰,提取的基因组DNA可满足PCR扩增及以PCR为基础的实验需要,可以应用于法庭科学实践。
Explore the effective method of extracting DNA from trace plant samples and try to apply it in forensic science practice. METHODS: DNA was extracted from 10 different plant leaves (8 common plants and 2 original drug plants) using the optimized modified silicon bead method. The extracted DNA was detected by ultraviolet spectrophotometry, agarose gel electrophoresis, PCR amplification and restriction endonuclease digestion. Finally, the actual case of the plant samples for the study to verify the extraction method. Results: The plant DNA extracted by this method has higher purity and better quality. PCR amplification of the clear, bright, no band. CONCLUSION: This method can effectively remove the interference of secondary biomass on DNA. The extracted genomic DNA can meet the needs of PCR amplification and PCR-based experiments and can be applied to forensic science practice.