丙酮酸分批发酵的供氧控制模式

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以一株光滑球拟酵母的多重维生素营养缺陷型为研究菌株,考察了分批发酵中不同体积传氧系数(kLa)对其产丙酮酸性能的影响.高kLa(450 h-1)下,丙酮酸产率较高(0.797 g@g-1),但葡萄糖消耗速度较慢(1.14 g@L-1@h-1);低kLa(200h-1)下,葡萄糖消耗速度快(1.97 g@L-1@h-1),然而丙酮酸产率(0.483 g@g-1)却明显下降.根据发酵过程主要参数和碳流分配的变化特性提出了发酵前16 h控制kLa为450 h-1、16 h后控制kLa为200 h-1的分阶段供氧
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