目的:建立5种升麻的薄层色谱指纹图谱,并进行化学计量学分析。方法:样品经甲醇超声提取,点样于硅胶G预制板上,以三氯甲烷-甲醇-水(13∶7∶2)10℃以下放置的下层溶液为展开剂展开,于紫外光灯(365 nm)下检视并成像,再喷以茴香醛试液,于105℃加热至条斑清晰,置紫外光灯(365 nm)下检视并成像。图像经ChemPattern软件转换成数码轮廓图并生成共有模式后,进行相似度、二维聚类和主成分分析。使用升麻素、升麻苷、升麻亭、阿魏酸等6种对照品和QTofMS进行成分识别。结果:紫外光灯(365 nm)下,升麻色谱可见9个明显的淡蓝色条斑,显色后再置紫外光灯(365 nm)下,可见16个不同颜色的条斑,正品与非正品区别明显,3种正品的指纹图谱较为接近。结论:薄层色谱指纹图谱分析法适用于升麻的快速鉴别和质量控制。 Objective: To establish the TLC fingerprints of five kinds of cimicifuga, and to carry out chemometric analysis. Methods: The sample was extracted by methanol and spotted on the prefabricated silica gel plate. The lower layer was placed under the chloroform (methanol) - water (13: 7: 2) nm) and then imaged with anisaldehyde solution. The solution was heated at 105 ° C until the plaque was clear and visualized under UV light (365 nm). After the images were converted into digital profiles by ChemPattern software and the common pattern was generated, the similarity, two-dimensional clustering and principal component analysis were performed. The use of cimicifugu, aconitine, ascending kiosk, ferulic acid and other six kinds of reference substance and QTofMS components identification. Results: Under visible light (365 nm), nine obvious light blue bands were observed in the color spectrum of cimicifuga, and 16 different color bands were observed under UV light (365 nm) Non-genuine difference is obvious, three genuine fingerprints are more similar. Conclusion: TLC fingerprinting is suitable for the rapid identification and quality control of cohosh.