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以澳洲坚果品种‘Renown’、‘Own choice’、‘HAES900’、‘HVA16’的接穗为供试材料。用6种剂量的60Co-γ射线辐射处理后嫁接,以未经辐照的接穗为对照。对嫁接成活率、植株生长变异情况进行调查分析。结果表明:在15~90 Gy辐照剂量范围内,随着辐射剂量加大,对嫁接成活率的抑制作用逐渐增强。不同品种的澳洲坚果对辐射的敏感性差异较大。4个品种(‘Own choice’、‘HVA16’、‘Renown’、‘HAES900’)枝条的辐射半致死剂量分别为19.68、18.23、18.04和10.81 Gy。本研究可为澳洲坚果辐射诱变育种适宜剂量的确定提供依据。
The scion of ’Renown’, ’Own choice’, ’HAES900’ and ’HVA16’ were used as test materials. Grafted with 6 doses of 60 Co-gamma radiation, the non-irradiated scion was used as a control. Survival rate of grafting, plant growth and variability were investigated. The results showed that the inhibitory effect on the graft survival rate gradually increased with the increase of radiation dose in the dose range of 15-90 Gy. Different varieties of macadamia differ greatly in their sensitivity to radiation. The radiation lethal doses of the four varieties (’Own choice’, ’HVA16’, ’Renown’, ’HAES900’) were 19.68, 18.23, 18.04 and 10.81 Gy, respectively. This study may provide the basis for the determination of appropriate dose of mutation breeding in macadamia nut.