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合成生物学的迅猛发展使其在各个领域得到了广泛应用,底盘设计、元件组装、代谢网络的从头构建、大片段DNA克隆、多片段DNA拼接等合成生物学技术的开发和利用大大提高了工业生物技术的竞争力.聚羟基脂肪酸酯(PHA)是一种具有生物可降解和生物相容性等优良特性的生物塑料,可以在许多细菌胞内合成,已经被开发应用于多个领域.但是,PHA高昂的生产成本阻碍了其大规模应用.基于合成生物学研究而得到的新方法、新技术可以改变细菌生长模式、生长条件以及细菌形态,从而进一步降低PHA的生产成本.另一方面,通过改造细菌基因组如弱化?-氧化途径可以得到不同种类的重组菌株,用于生产具有不同性能的包括无规共聚物、嵌段共聚物、带有官能团的聚合物等在内的新型多功能PHA材料.合成生物学的应用开创了低成本、高附加值的PHA材料生产的新时代,为PHA的产业化奠定了坚实的基础.
The rapid development of synthetic biology has made it widely used in various fields. The development and utilization of synthetic biology technologies such as chassis design, assembly of components, rebuilding of metabolic networks, large DNA cloning and multi-segment DNA splicing have greatly enhanced the industrial Biocompatibility Polyhydroxyalkanoates (PHAs) are bioplastics with excellent biodegradability and biocompatibility that can be synthesized intracellularly in many bacteria and have been developed and applied in many fields. However, the high production costs of PHA have hindered its large-scale application.Based on the new methods of synthetic biology research, new technologies can change the growth mode of bacteria, growth conditions and bacterial morphology to further reduce the production cost of PHA, on the other hand , Different types of recombinant strains can be obtained by modifying the bacterial genome such as the attenuated? -oxidation pathway for the production of novel multi-functional products including random copolymers, block copolymers, functional group-containing polymers and the like PHA materials. The application of synthetic biology opens up a new era of low cost, high value-added PHA material production for PHA Industry laid a solid foundation.